Fig. 3: Identification of miRNAs transcriptionally regulating the expression of NDRG2. | Cell Death & Disease

Fig. 3: Identification of miRNAs transcriptionally regulating the expression of NDRG2.

From: The MYB/miR-130a/NDRG2 axis modulates tumor proliferation and metastatic potential in salivary adenoid cystic carcinoma

Fig. 3

a Hierarchical clustering of gene expression values of the 176 dysregulated miRNAs in the tumor and normal salivary gland samples (log2FC > 1.5 or <−1.5, FDR < 0.05) were charted exhibiting the miRNA expression levels. b The abundance of the 3 candidate miRNAs potentially regulating NDRG2 mRNA. NDRG2 was detected by micronome profiling. c NDRG2 protein levels were assessed after transfecting the miR-130a inhibitor, miR-181a inhibitor, miR-324-3p inhibitor or inhibitor control into SACC-LM cells. d NDRG2 protein levels were assessed after transfecting the miR-130a mimics, miR-181a mimics, miR-324-3p mimics or mimic control into SACC-LM cells. e MiR-130a downregulated NDRG2 in SACC-83 (left) and SACC-LM (right) cells in a dose-dependent manner. f The wide-type NDRG2 3′UTR sequence and the mutant NDRG2 3′UTR sequence were inserted into the luciferase reporter vector. g Forced expression of miR-130a significantly diminished the luciferase reporter activity of the pSiCheck™-2-NDRG2-3′UTR (P < 0.01), but not pSiCheck™-2-NDRG2-mutant 3′UTR compared with the miR-con

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