Fig. 4
a The relative expression of miR-130a in 21 pairs of SACC tumors and the adjacent normal salivary glands. b The mRNA relative expression of miR-130a was inversely correlated with NDRG2 in the 21 samples. c Effect of miR-130a overexpression on SACC-83 and SACC-LM cell growth. d Clonogenic assays showed that miR-130a overexpression significantly increased the colony-forming ability. e MiR-130a overexpression enhanced SACC-83 cell migration (magnification ×400). f Transwell assay showing that miR-130a overexpression promoted cell invasion in SACC-83 and SACC-LM cells (magnification ×200). g Tumor volumes were compared between xenograft tumors (n = 5) induced with SACC-LM-130a-con and SACC-LM-miR-con cells. h Images of xenograft tumors were shown for the indicated miR-130a-overexpressing and miR-con cells. The weight of the tumors was analyzed. i Lung metastasis experiments were performed in mice using lenti-miR-130a cells or miR-con cells. The tumor volume and the bilateral lung weight were analyzed. j The histopathologic analysis, by HE staining, of metastases induced by SACC-LM-con and SACC-LM-130a cells in lung tissues (original magnifications ×100). (* necrosis)
