Fig. 5: Re-expression NDRG2 reversed the miR-130a-imposed effects on SACC cell proliferation and metastasis in vitro.

a NDRG2 protein expression was increased by the overexpression of NDRG2 in miR-130a-overexpressing cells. b A CCK8 assay showed that NDRG2 overexpression significantly decreased the proliferative ability of miR-130a-transfected SACC cells. c Clonogenic assays showed that NDRG2 significantly limited the colony-forming ability of miR-130a- overexpressing cells relative to the that of the negative control. d Wound-healing assay indicated that NDRG2 significantly decreased cell motility of miR-130a-overexpresing cells. e Transwell assays revealed that NDRG2 significantly suppressed the invasion of miR-130a-transfected cells