Fig. 6: MYB upregulated miR-130a expression directly and induces low protein levels of NDRG2 | Cell Death & Disease

Fig. 6: MYB upregulated miR-130a expression directly and induces low protein levels of NDRG2

From: The MYB/miR-130a/NDRG2 axis modulates tumor proliferation and metastatic potential in salivary adenoid cystic carcinoma

Fig. 6

a The protein level of MYB in SACC cells was greatly reduced after transfecting either siMYB. The protein amounts of NDRG2 were significantly increased when MYB was silenced. b MiR-130a expression was downregulated in SACC cells when MYB was silenced. c Genomic structure of miR-130a: the promoter region was depicted in orange and the pri-miR-130a encoding region was shown in gray. The six potential binding sites were in black. A luciferase reporter assay was conducted in SACC-83 cells (d) and SACC-LM (e) cells transfected with the PGL3 plasmid constructs with promoter fragment 1 (containing site 1, site 2 and site 3) or promoter fragment 2 (containing site 4, site 5 and site 6) in the presence or absence of pCDNA3.1-MYB. The ChIP qPCR assay displayed that the recruited amounts of MYB binding to either site 1, site 2, site 3, site 5, or site 6 in the miR-130a promoter region were significantly higher than that of negative control IgG in SACC-83 f and SACC-LM g cells

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