Fig. 3: EIF5A1 regulates ARAF expression in trophoblasts through directly binding to its mRNA. | Cell Death & Disease

Fig. 3: EIF5A1 regulates ARAF expression in trophoblasts through directly binding to its mRNA.

From: EIF5A1 promotes trophoblast migration and invasion via ARAF-mediated activation of the integrin/ERK signaling pathway

Fig. 3

a, b Heatmap (a) and volcano plot (b) showing differentially expressed proteins after GC7 treatment. C1–3 indicates control samples, T1–3 indicate treated samples. c Signaling pathways enriched following GC7 treatment based on PANTHER analyses. d Heatmap showing expression of the 10 dysregulated proteins from the integrin signaling pathway. e, f The effects of GC7 (e) and siEIF5A1 (f) treatment on ARAF protein expression in HTR-8 cells as evaluated by western blotting. g Western blot showing the effects of EIF5A1 upregulation or GC7 treatment on ARAF expression in naive HTR-8 or EIF5A1-overexpressing HTR-8 cells. h IF staining of ARAF in HTR-8 cells treated with GC7, siEIF5A1, EIF5A1, EIF5A1 + GC7 and their controls. Original magnification × 200, scale bar = 25 μm. i IF staining of ARAF in trophoblasts of villous explants treated with GC7, siEIF5A1, EIF5A1, EIF5A1 + GC7 and their controls. Original magnification × 200, scale bar = 25 μm. j-l RIP assays from HTR-8 cells treated with GC7, siEIF5A1, EIF5A1, EIF5A1 + GC7 and EIF5A1K50A. The cell lysates were incubated with either the EIF5A1 antibody or IgG. **P < 0.01, ***P < 0.001

Back to article page