Table 2 List of primers used in RT-PCR
Target Gene | sequence (5′ to 3′) | Annealing temperature (°C) | product size (bp) |
|---|---|---|---|
Bax | GGGGACGAACTGGACAGTAA | 50 | 490 |
CCTCCCAGAAAAATGCCATA | |||
Bcl2 | GGATGCCTTTGTGGAACTGT | 50 | 479 |
GGTGCTTGGCAATTAGTGGT | |||
Caspase 3 | TGGAATTGATGCGTGATGTT | 84 | 460 |
TCAAGCTTGTCGGCATACTG | |||
Caspase 9 | GCTTAGGGTCGCTAATGCTG | 25 | 490 |
GTGCTGAACATCCCACAATG | |||
GAPDH | GTCAGGTCCACCACTGACAC | 55 | 225 |
GGAAGGACTCATGACCACAG | |||
LC3B | GGTGAGAAGCAGCTTCCTGT | 50 | 235 |
TCTCCTGGGAGGCATAGACC | |||
Beclin1 | GGACACTCAGCTCAACGTCA | 45 | 208 |
AGCCTGGACCTTCTCGAGAT | |||
Atg3 | GAGCAACGGCAGCCTTTAAC | 25 | 192 |
TCCAAGTTCTCCCCCTCCTT | |||
Atg5 | ACAGATGACAAAGATGTGCT | 50 | 228 |
TGGTGTGCCTTCATATTCAA | |||
Atg7 | TGCTATCCTGCCCTCTGTCT | 52 | 193 |
GCAAGGAAACCAGCACCATG | |||
Atg12 | AAGTGGGCAGTAGAGCGAAC | 52 | 203 |
CACGCCTGAGACTTGCAGTA | |||
E-Cadherin | TCATGAGTGTCCCCCGGTAT | 50 | 240 |
TCTTGAAGCGATTGCCCCAT | |||
Snail | GAGGACAGTGGGAAAGGCTC | 52 | 248 |
TGGCTTCGGATGTGCATCTT | |||
Slug | CATCTTTGGGGCGAGTGAGT | 52 | 191 |
GGCCAGCCCAGAAAAAGTTG |
