Fig. 2

Knockout of PAR2 reduced YAP protein in vivo. a Protein and (b) mRNA expression of YAP in mouse colon was detected with western blotting or RT-PCR, respectively. c Immunohistochemical analysis of paraffin-embedded colon sections from wild-type and PAR2 knock-out mice. Scale bars represent 100 μm. d Number of colon epithelial cell with nuclear YAP was quantified. Data are shown as mean ± SEM. N.S., no significance; ***p < 0.001 vs. corresponding control