Fig. 6 | Cell Death & Disease

Fig. 6

From: Protease-activated receptor 2 signaling modulates susceptibility of colonic epithelium to injury through stabilization of YAP in vivo

Fig. 6

PP1-mediated PAR2 activation induced dephosphorylation of YAP. a Whole-cell lysate of HT-29 was immunoprecipitated with anti-YAP antibody or control IgG, followed by western blot for PP1, PP2a, PP2Ab, and PP2Ac. b HEK293 cells were treated with PAR2-AP for 30 min. Then, cell lysates were collected and used for immunoprecipitation with anti-YAP antibody. PP1 were detected by western blot. c Twenty-four hours after transfection with two different siRNAs against PP1, HEK293 cells were serum-starved for 12 h and then stimulated with PAR2-AP for 30 min. Western blot was used to determine p397-YAP and YAP. d The density of WB bands from panel d was analyzed by image-pro-plus soft system. Fold change of YAP phosphorylation is calculated as [p397-YAP /YAP]PAR2-AP/[p397-YAP /YAP]NC. Data were shown as mean ± SEM. *p < 0.05 vs. NC control. e Diagram of signaling pathways responsible for PAR2-regulated YAP stability and function

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