Fig. 2: Hepcidin secretion in TC cells is modulated by SOSTDC1. | Cell Death & Disease

Fig. 2: Hepcidin secretion in TC cells is modulated by SOSTDC1.

From: E4BP4 promotes thyroid cancer proliferation by modulating iron homeostasis through repression of hepcidin

Fig. 2

a The concentration of BMP4, BMP7, IL-6 in the serum of TC patients (n = 48) and healthy participants (n = 38). b The secretion of hepcidin in the indicated TC cells treated with antibodies of IL-6 or IL-6 for 24 h. c The secretion of hepcidin in the indicated TC cells treated with BMP4, BMP7, or their combination for 24 h. d The intracellular labile iron contents in indicated TC cells treated as in (c). e Proliferation of cells treated in (c) was analyzed by MTT assay. f Proliferation of cells treated in (c) was analyzed by BrdU assay. g Left, protein expressions in K1 and 8505C cells transfected by SOSTDC1 plasmid; right, the relative density of p-SMAD vs. SMAD was analyzed by Image J software. h The secretion of hepcidin in indicated TC cells transfected with SOSTDC1 plasmid. i The intracellular labile iron contents in in indicated TC cells transfected with SOSTDC1 plasmid. j The proliferation of K1 cells transfected with SOSTDC1 plasmid was analyzed by MTT assay. k The proliferation of K1 cells transfected with SOSTDC1 plasmid was analyzed by BrdU assay. Each experiment was repeated for three times. NS P > 0.05, *P < 0.05, **P < 0.01

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