Fig. 3: The methylation of SOSTDC1 promoter suppressed its expression. | Cell Death & Disease

Fig. 3: The methylation of SOSTDC1 promoter suppressed its expression.

From: E4BP4 promotes thyroid cancer proliferation by modulating iron homeostasis through repression of hepcidin

Fig. 3

a SOSTC1 expression in TC from TCGA database. b mRNA levels of SOSTDC1 in indicated cell lines. c Upper, protein levels of SOSTDC1 in indicated cell lines; lower, the relative density was analyzed by image J software. d Bisulfite-modified genomic DNA without promoter hypermethylation of SOSTDC1 was analyzed by methylation-specific PCR. e The genomic DNA from indicated cells with or without 5-Aza. f Protein expressions of SOSTDC1 in TC cells with or without 5-Aza. g mRNA expression of SOSTDC1 in TC cells with or without 5-Aza. h The secretion of hepcidin in TC cells with or without 5-Aza. i The proliferation TC cells with or without 5-Aza was analyzed by MTT assay. j The proliferation TC cells with or without 5-Aza was analyzed by BrdU assay. Each experiment was repeated for three times. *P < 0.05, **P < 0.01, ***P < 0.001

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