Fig. 5: E4BP4 recruits G9a into SOSTDC1 promoter in TC cells. | Cell Death & Disease

Fig. 5: E4BP4 recruits G9a into SOSTDC1 promoter in TC cells.

From: E4BP4 promotes thyroid cancer proliferation by modulating iron homeostasis through repression of hepcidin

Fig. 5

a Interaction of G9a, DNMT1 and E4BP4 analyzed by immunoprecipitation (IP) with anti-G9a antibody. b Interaction of G9a, DNMT1, and E4BP4 analyzed by immunoprecipitation (IP) with anti-E4BP4 antibody. c The binding of G9a and SOSTDC1 promoter in K1 cells transfected by control or E4BP4 shRNAs. d The binding of E4BP4 and SOSTDC1 promoter in K1 cells transfected by control or G9a siRNAs. e The immunofluorescence of G9a in K1 cells transfected by control or E4BP4 shRNAs. The IgG staining was used as negative control. Scale bar, 10 µm. f The western blot of G9a in cytosolic and nuclear fraction of K1 cells transfected with control or E4BP4 shRNAs. g mRNA expression of SOSTDC1 in K1 and 8505 C cells transfected by control or E4BP4 shRNAs. h Secretion of hepcidin in K1 and 8505C cells transfected by control or E4BP4 shRNAs. i The proliferation of K1 and 8505C cells transfected by control or E4BP4 shRNAs. Each experiment was repeated for three times. *P < 0.05; **P < 0.01

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