Fig. 6: Absence of E4BP4 limits the TC proliferation in vivo.

a 1 × 106 K1 cells stably transfected with control or E4BP4 shRNA was injected into nude mice to generate xenograft tumors. The growth of tumor was shown. b The representative tumors in control or E4BP4 shRNA-transfected group. c The expression of indicated proteins in pool samples from control or E4BP4 shRNA-transfected tumors. d The mRNA level of SOSTDC1 in control or E4BP4 shRNA-transfected tumors. e The hepcidin mRNA in control or E4BP4 shRNA-transfected tumors. f The immunochemistry staining of Ki-67 in control or E4BP4 shRNA-transfected tumors. Scale bar, 20 µm. g The immunofluorescence staining of G9a in control or E4BP4 shRNA-transfected tumors. Scale bar, 10 µm. Each experiment was repeated for three times. *P < 0.05, ***P < 0.001