Fig. 3: Inhibition of PYK2 kinase activity affects TAZ level and YAP Ser127 phosphorylation in TNBC cell lines.
From: PYK2 negatively regulates the Hippo pathway in TNBC by stabilizing TAZ protein
a The indicated TNBC cell lines were treated for 5 h with ascending concentrations of a dual PYK2/FAK (PF396) or a selective FAK (PF228) inhibitor. TAZ protein level and YAP Ser127 phosphorylation were determined by WB analysis. The correlating graphs show a quantified presentation of the WB results as fold of control. b Subcellular localization of YAP in cells treated as indicated was examined by IF analysis. Shown are representative results obtained in HCC1937 (YAP red) or Hs578T (YAP green) cells. Scale bar: 10 μm. c The percentage of cells with nuclear (N) or cytosolic (C) YAP-staining was quantified based on the criteria shown below the graph. (mean; n = 3). d HEK293T cells were transfected with HA-PYK2 and its influence on endogenous TAZ was determined in the presence or absence of PYK2/FAK inhibitors. Quantification shown below in bar graphs as fold of control
