Fig. 3
From: Linc02527 promoted autophagy in Intrahepatic cholestasis of pregnancy
Linc02527 regulated autophagy and proliferate in HTR8 cells. a The apoptosis rate was detected using flow cytometry when silencing Linc02527. b The expression of LC3-II, BCL-2 and BAX was detected by western blot after silencing Linc02527.(c)The apoptosis rate was detected using flow cytometry when ectopically expression of Linc02527. d The expression of LC3-II, BCL-2 and BAX was detected by western blot when ectopically expression of Linc02527. e The expression of LC3-II was detected by western blot. LV5-NC: HTR8 cells infected by LV5-NC. LV5-NC + CQ: HTR8 cells infected by LV5-NC, then adding chloroquine at 10uM for 24 h. LV5: HTR8 cells infected by LV5-lnc02527. LV5 + CQ: HTR8 cells infected by LV5- lnc02527, then adding chloroquine at 10 uM for 24 h. f The expression of LC3 in placenta of ICP and normal group was detected by western blot. g–j The cellular proliferation was detected by EdU. k The location of Linc02527 was determined hybridization in situ. Lnc02527 probe was detected the location of Linc02527. NC was negative control. Error bars represented standard error. Asterisks(*) and ** indicated p < 0.05 and 0.001, respectively
