Fig. 4

The expression of miR-3185 was regulated by Linc02527. a The expression of Linc02527 was determined by qPCR. b The miRNAs expression was detected by qPCR in HTR8 cells infected LV5-lnc02527 or LV5-NC. c The potential binding sites between Linc02527 and miR-3185 were predicted. d HTR8 cells were co-transfected with miR-3185 mimic or control RNA (NC) with luciferase reporter plasmids containing either wild-type (pMIR-lnc02527) or mutant (pMIR-lnc02527m) of Linc02527 genes. Luciferase expression was measured. The fold changes of the relative luciferase activity in miR-3185 mimic indicated that plasmid transfected cells were normalized to NC, indicating plasmid transfected cells. e The expression of Linc02527 was detected by transfecting miR-3185 mimic or NC(negative control). f The expression of Linc02527 was detected by transfecting miR-3185 inhibitor or NC(negative control). Error bars represented standard error. Asterisks(*) and ** indicated p < 0.05 and 0.001, respectively