Fig. 5
From: Linc02527 promoted autophagy in Intrahepatic cholestasis of pregnancy
The expression of ATG5 and ATG7 was regulated by miR-3185. a The expression of ATG5 mRNA was detected when transfecting with miR-3185 mimic, miR-3185 mimic control, miR-3185 inhibitor or miR-3185 inhibitor mimic. b The expression of ATG7 mRNA was detected when transfecting with miR-3185 mimic, miR-3185 mimic control, miR-3185 inhibitor or miR-3185 inhibitor mimic. c The protein expression of ATG5, ATG7 and LC3-II was determined when transfecting with miR-3185 mimic, miR-3185 mimic control, miR-3185 inhibitor or miR-3185 inhibitor mimic. d, e The potential binding sites between miR-3185, ATG5 and ATG7 were predicted. f, g HTR8 cells were co-transfected with miR-3185 mimic or control RNA (NC) with luciferase reporter plasmids containing either wild-type (pMIR-ATG5-3’UTR and pMIR-ATG7-3’UTR) or mutant (pMIR-ATG5-3’UTRm and pMIR-ATG7-3’UTRm) of ATG5 and ATG7 genes. Luciferase expression was measured. The fold changes of the relative luciferase activity in miR-3185 mimic indicated that plasmid transfected cells were normalized to NC, indicating plasmid transfected cells. h, i The expression of ATG5 and ATG7 was determined in HTR8 cells infected with LV5-NC or LV5-lnc02527. j, k The expression of ATG5 and ATG7 was determined in HTR8 cells infected with LV3-NC, LV2-lnc02527-1 or LV2-lnc02527-2. Error bars represented standard error. Asterisks(*) and **indicated p < 0.05 and 0.001, respectively
