Fig. 6
From: Linc02527 promoted autophagy in Intrahepatic cholestasis of pregnancy
Linc02527 directly binding to YBX1 and activated P21. a Silver stains for protein gels obtained by Linc02527 RNA Pulldown. Bio-lnc02527 (sense): treatment group; Bio-lnc02527 (antisense). The Linc02527 specific binding protein gels (in red box) was identified by MALDI-TOF-MS. Results show that YBX1 was a direct binding protein to Linc02527. b, c YBX1 was identified by MALDI-TOF-MS. d The expression of LC3-II,YBX1 and P21 was determined in HTR8 cells infected with LV5-NC or LV5-lnc02527. e The expression of LC3-II,YBX1 and P21 was determined in HTR8 cells infected with LV3-NC, LV2-lnc02527-1 or LV2-lnc02527-2. f The Linc02527 specific binding protein gels was identified by RNA pulldown. FL was the biotin Linc02527. NC was the Linc02527 without biotin label
