Fig. 4: MiR-873-5p regulates hepatocyte apoptosis and cholangiocyte proliferation by targeting GNMT.
From: MiR-873-5p acts as an epigenetic regulator in early stages of liver fibrosis and cirrhosis
(a) Gnmt and miR-873-5p expression in cultured hepatocytes treated with deoxycholic acid (DCA) for 2 h (N = 3). (b) GNMT expression analysis by qPCR and WB after anti-miR-873-5p and DCA treatment. Cell death was measured by C3 activity (2 h) TUNEL and JNK phosphorylation (15’/30’) at different time points of DCA treatment (c, d). (e) Gene expression analysis by qPCR of indicated genes implicated in BA-induced apoptosis, metabolism and transport after 2 h of DCA and anti-miR.873-5p treatment (N = 3). Densitometry analyses of WB are shown in Supp. Fig. 5A. (f) MiR-873-5p expression in normal human cholangiocytes (NHC) after 48 h of Interleukin-6 (IL-6) treatment (N = 6). Cell proliferation measured by MTT assay and analysis of GNMT and indicated genes measured by qPCR in anti-miR-873-5p cholangiocytes treated with IL-6 (N = 4). Data normalized as fold change vs. control. Error bars represent the means ± SEM. Statistical significance was determined by the Student’s t-test or ANOVA when more than two groups were compared. p < 0.05*; p < 0.01**; p < 0.001***
