Fig. 1: PGAM5 expression in HCC tissues and its prognostic significance in patients with HCC.

a Representative immunohistochemistry staining of PGAM5 in HCC tissues and in adjacent non-neoplastic liver tissue (Original magnification: Left X100; Right X400). b PGAM5 was significantly up-regulated in HCC samples of both testing cohort (TMUCH, left, p < 0.01; Mann–Whitney test) and validation cohort (SYSUCC, right, p < 0.01; Mann Whitney test). c Western blotting showing higher PGAM5 levels in HCC fresh tissues than in paired adjacent non-neoplastic hepatic tissues in all 7 cases (T, hepatocellular carcinoma tissue; N, non-neoplastic hepatic tissues). d, e Kaplan-Meier survival analysis show that higher PGAM5 expression predicts poorer Overall Survival (d) and progression-Free survival (e) in 178 HCC patients of the testing cohort (left) and in 212 cases of the validation cohort(right)