Fig. 2: Diets alter tissue levels of VitC, VitE, and superoxide after blast. | Cell Death & Disease

Fig. 2: Diets alter tissue levels of VitC, VitE, and superoxide after blast.

From: Antioxidants prevent inflammation and preserve the optic projection and visual function in experimental neurotrauma

Fig. 2

a Quantification of retina ascorbic acid (VitC) levels. Retinas of Gulo-/- mice provided a low-VitC content diet had less ascorbic acid, *p < 0.05. Retinas of wild-type mice fed a high-VitE diet contained normal levels of ascorbic acid. b Quantification of retina α-tocopherol (VitE) levels. Retinas of wild-type mice fed a high-VitE diet contained higher levels of α-tocopherol. c, d Representative images of DHE fluorescence (superoxide levels) in the retinas of sham (c) and repeat blast-exposed (d) mice. e Quantification of retina DHE fluorescence in normal, low-VitC, and high-VitE-diet mice. Levels are increased in the 2 and 4 weeks after blast injury in both the control and low-VitC mice, but not in the high-VitE mice. f Western blots of SOD2 levels in retinas from sham, and 2- or 4-week post-blast mice on control, low-VitC, or high-VitE diets. g Quantification of SOD2 levels. SOD2 levels are decreased at 4 weeks after injury in both normal-diet and low-VitC-diet mice, but not the high-VitE mice. Low VitC retinas contained increased levels of SOD2 in sham and 2 week post-blast mice, suggesting an endogenous compensatory effect of the low-VitC diet. This experiment was repeated twice. n = 5 for all groups. *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.0001

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