Fig. 3: Diets alter activation of the inflammasome pathway after injury. | Cell Death & Disease

Fig. 3: Diets alter activation of the inflammasome pathway after injury.

From: Antioxidants prevent inflammation and preserve the optic projection and visual function in experimental neurotrauma

Fig. 3

a Western blots of cleaved and total caspase-1 in sham and post-blast mice treated with a high-VitE or low-VitC diet. b Quantification of cleaved to total caspase-1 shows a greater increase in low-VitC retinas than control-diet mice. There was no increase in activated caspase -1 in the retinas of mice on a high-VitE diet. c, d Quantification of IL-1α (c) and IL-1β (d) shows that levels are elevated in retinas of mice on a low-VitC diet regardless of injury. There was no increase in IL-1α or IL-1β levels in the retinas of high-VitE-diet mice. e Quantification of IL-18 levels also shows an overall elevation in retinas from mice on a low-VitC diet. In addition, these mice also had a transient increase at 2 weeks after injury as in control-diet mice. There was no increase in IL-18 in the retinas from the high-VitE-diet mice. This experiment was repeated twice. n = 5 for all groups. *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.0001

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