Fig. 1: UBCS039 induces deacetylation of SIRT6-targeted histone H3 sites in human H1299 cells.
From: Pharmacological activation of SIRT6 triggers lethal autophagy in human cancer cells
a, b Western blot analysis of acetylation levels of histone H3K9 and H3K56 in H1299 human non-small cell lung cancer cell line treated with 75 µM of SIRT6 activator UBCS039 (a) or of the chemical analog UBCS060 (b) for the indicated times. H3 acetylation levels were quantified by densitometric analyses using ImageJ software and the relative levels of histone modifications were expressed in the histograms as fold changes of treated versus untreated samples, after H3 normalization. The results represent mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001
