Fig. 2: UBCS039 induces autophagosome accumulation.
From: Pharmacological activation of SIRT6 triggers lethal autophagy in human cancer cells
a, b Western blot analysis of microtubule-associated protein 1 form II (LC3B-II) protein level in H1299 (a) and HeLa (b) cells treated with 75 µM UBCS039 for the indicated times. LC3B-II levels were quantified by densitometric analyses using ImageJ software and the relative levels of LC3B-II were expressed in the histograms as fold changes of treated versus untreated samples, after HSP70 normalization. *P < 0.05; **P < 0.01. S.E. short exposure, L.E. long exposure. c Representative images and relative quantification of fluorescence microscopic analysis of LC3B puncta positive cells in H1299 EGFP-LC3B treated as indicated in a. Olympus AX70 microscope, ×100 magnification. Scale bar indicates 10 µm. The results represent the mean ± SEM of three independent experiments. **P < 0.01
