Fig. 5: UBCS039 induces autophagy via AMP-activated protein kinase (AMPK) signaling pathway activation.
From: Pharmacological activation of SIRT6 triggers lethal autophagy in human cancer cells
a, b Representative experiment of flow cytometric analysis of ROS production by DHE staining in H1299 (a) and HeLa (b) cell lines treated with 100 µM UBCS039 for the indicated times. ROS production was expressed in the histograms as percentage of cells positive for DHE staining. Results represent mean ± SD of three independent experiments. ***P < 0.001. c Western blot analysis of LC3B-II protein levels in HeLa cells treated with 100 µM UBCS039 in combination or not with antioxidant compounds NAC (5 mM) and Trolox (200 µM) for the indicated times. LC3B-II levels were quantified by densitometric analyses using ImageJ software and the relative levels of LC3B-II were expressed in the histograms as fold changes of treated versus untreated samples, after β-actin normalization. Results represent mean ± SD of three independent experiments. **P < 0.01; ***P < 0.001; ****P < 0.0001. d Western blot analysis of the indicated proteins in HeLa cells treated with 100 µM UBCS039 for the indicated times. Total AMPK, total ULK1, total mTOR, and HSP70 were used as internal loading controls
