Fig. 6: UBCS039 leads to autophagy-mediated apoptosis.
From: Pharmacological activation of SIRT6 triggers lethal autophagy in human cancer cells
a In vitro growth curves of H1299 and HeLa cell lines treated or not with UBCS039 or UBCS060 at the indicated doses. In all, 5 × 104 cells were seeded at day 0 and treated at day 1. The figure represents mean ± SD of three independent experiments. b Representative experiment of flow cytometric analysis of AnnexinV-positive cells in H1299 and HeLa cell lines treated with 100 µM UBCS039 for 72 h in combination or not with pan-caspase inhibitor zVAD-fmk (50 μM) or the late-stage autophagy inhibitor CQ (25 μM). The results represent mean ± SD of three independent experiments. c Representative experiment of flow cytometric analysis of AnnexinV-positive cells in HeLa human epithelial cervix carcinoma cell lines transiently transfected with the empty vector (SCR) and with the wild-type (WT) or the catalytically inactive form of SIRT6 protein (H133Y) for 72 h. The results represent mean ± SD of three independent experiments. *P < 0.05; **P < 0.01
