Fig. 1: Adult Mkk4- and Mkk7-deficient retinas exhibit presence of all major cell types with minor structural alterations. | Cell Death & Disease

Fig. 1: Adult Mkk4- and Mkk7-deficient retinas exhibit presence of all major cell types with minor structural alterations.

From: Mkk4 and Mkk7 are important for retinal development and axonal injury-induced retinal ganglion cell death

Fig. 1

a Representative 3ā€‰Ī¼m plastic sections stained with H&E demonstrate areas of photoreceptor cell body attachment to retinal pigmented epithelium in Mkk4-deficient retinas (arrowheads). Misplaced cells are also seen in the inner plexiform layer in Mkk4-deficient retinas. Retinal lamination in Mkk7-deficient retinas appeared grossly normal, though regions of the optic nerve head displayed slightly increased cellularity in the prelaminar region. Antibodies against ChAT and calretinin revealed normal development of cholinergic and AII amacrine cells, respectively, in both Mkk4- and Mkk7-deficient retinas (b). Calbindin-D-28K-labeled horizontal cells (c), PKCĪ±-labeled bipolar cells (d), and SOX2-labeled MĆ¼ller glia (e) in Mkk4 and Mkk7 mutants were furthermore indistinguishable from controls. Merged images with DAPI (blue) are shown below.Ā Nā€‰ā‰„ā€‰4 retinas for each genotype. Scale bars: 100ā€‰Ī¼m

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