Fig. 6: Fasciculation of RGCs and amacrine cells in retinas of Mkk4/Mkk7 double-deficient mice.

Retinal flat mounts were stained with neurofilament (NF; a) and TUJ-1 (b) to assess RGC organization. Mkk4/Mkk7 double-deficient mutants displayed phenotypic variability in RGC structure, in which some areas exhibited axonal overlap (arrowheads in a’) and irregular axonal trajectories (b’), other areas appeared relatively normal (a”), and several areas consisted of RGC clumps (a”’, b”’). N ≥ 4 per genotype. Scale bars: 100 μm