Fig. 4: Cell cycle genes are upregulated in SMA motor neurons.
From: Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy
a Motor neurons were purified based on HB9 immunoreactivity. mRNA expression levels of CDKs and cyclins measured by RNA-seq and qPCR respectively. Graph shows fold change comparing SMA HB9+ motor neurons to wild-type HB9+ motor neurons. The blue dotted line indicates relative expression of wild-type HB9+ motor neurons. b qPCR analysis of ISL1+ motor neurons derived from day 28 organoids. Graph shows mRNA fold change, relative to BJ ISL1+ motor neurons. c Knockdown of SMN in wild-type motor neuron cultures revealed upregulation of cell cycle genes. *p < 0.05; **p < 0.01, and ***p < 0.001
