Fig. 2: Plasminogen is needed for the histological changes in the skin after irradiation.
From: Plasminogen activation is required for the development of radiation-induced dermatitis
a Representative images of hematoxylin and eosin (H&E)-stained skin sections from plg+/+ and plg−/− mice before and on day 9 after irradiation. Arrowheads and arrows point to the epidermis in plg+/+ and plg−/− mice, respectively. b The thickness of the epidermis in plg+/+ and plg−/− mice on different days after irradiation measured in H&E-stained skin sections. c Representative images of skin sections stained for CD31 (red) from plg+/+ and plg−/− mice on day 12 after irradiation. Arrowheads and arrows point to the blood vessels in plg+/+ and plg−/− mice, respectively. d Quantification of blood vessels in plg+/+ and plg−/− mice at different time points after irradiation. e Representative images of Ki67-stained (red) skin sections from plg+/+ and plg−/− mice on day 12 after irradiation. Arrowheads and arrows point to the proliferating cells in plg+/+ and plg−/− mice, respectively. f Quantification of proliferating cells in plg+/+ and plg−/− mice at different time points after irradiation. g Representative images of TUNEL (green) staining of skin sections from plg+/+ and plg−/− mice on day 1 and day 12 after irradiation. Arrowheads and arrows point to the apoptotic cells in plg+/+ and plg−/− mice, respectively. h Quantification of apoptotic cells in plg+/+ and plg−/− mice at different time points after irradiation. i Representative images of 8-oxo-2′-deoxyguanosine staining of skin sections from plg+/+ and plg−/− mice on day 1 and day 12 after irradiation. Arrowheads and arrows point to cells with oxidized DNA in plg+/+ and plg−/− mice, respectively. j Quantification of 8-oxo-2′-deoxyguanosine in plg+/+ and plg−/− mice at different time points after irradiation. For all quantifications, n ≥ 3 per genotype. In all immunostained sections, DAPI (blue) was used for staining the nuclei. Scale bar = 100 µm. *P < 0.05; **P < 0.01.
