Fig. 6: The effect of BECN1 and ATG5 siRNAs on proliferation and apoptosis of RACK1 OE colon cancer cells.
From: RACK1 promotes tumorigenicity of colon cancer by inducing cell autophagy
a Western blot analysis showing the expression levels of BECN1, ATG5, and LC3-II in the SW480-RACK1 OE cells transfected with BECN1 or ATG5 siRNA and control cells. Analysis of cell proliferation by CCK-8 (b), plate clone formation(c) and EdU incorporation (d) assay in the SW480-RACK1 OE cells transfected with BECN1 or ATG5 siRNA and control cells. Analysis of cell cycle distribution (e) and cell apoptosis (f) by flow cytometry in the SW480-RACK1 OE cells transfected with BECN1 or ATG5 siRNA and control cells. Three experiments were done; Means, SDs, and statistical significance are denoted; *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance. Scale bars = 200 μm. siBECN1, BENC1 siRNA; siATG5, ATG5 siRNA; siNC, negative control siRNA
