Fig. 1

miR-6775-3p is associated with good prognosis of ESCC and inhibits ESCC tumor growth and metastasis in vivo. a Gene structure of miR-6775 in the chr16 (q24.2) region of human genome. b Kaplan–Meier analysis revealed that high expression of miR-6775-3p is associated with better survival of ESCC patients. c–f, TE1 cells (5 × 10⁶ cells per mouse, n = 10 for each group) transfected with miR-6775-3p agomir or NC agomir transfection were subcutaneously injected into BALB/c nude mice. After cell injection, tumors were injected with miR-6775-3p agomir (totally 5 nM per mouse) or NC agomir (totally 5 nM per mouse) per 2 days. Tumor growth and tumor weight were analyzed. *P < 0.05. g, h, The expression of Ki67, PCNA, MMP2 and MMP9 in mice tumor tissues was examined by IHC. Bars: 50 μm. **P < 0.01, *P < 0.05. i, j TE1 cells (5 × 10⁶ cells per mouse, n = 10 for each group) transfected with miR-6775-3p agomir or NC agomir transfection were slowly injected under the splenic capsule of BALB/c nude mice. Five minutes after injection, the spleen was removed. Three weeks later, mice were sacrificed and the livers were initially examined by the naked eye, and HE staining was performed. The metastatic counts of each slice were observed. Bars: (left) 100 μm; (right) 50 μm. **P < 0.01