Fig. 2: Sublethal UV irradiation induces squamous differentiation in epidermal keratinocytes.
Primary human epidermal keratinocytes 24 h (d) or 48 h (a–c, e) after sublethal UV irradiation (sbUV; 15 mJ/cm2). a Representative flow-cytometry analyses for morphology (light scatter) or the expression of keratin K1. Black boxes gate cells with high light scatter typical of differentiation. b Percent of high scatter cells or keratin K1 positive cells. c Percent of involucrin (Invol) or keratin K13 positive cells. d Expression of K1, K10, filaggrin or Invol as measured by RT-qPCR. e Clonogenic capacity of cells plated 48 h after UV irradiation as in (a) (3000 cells per well in triplicates). NI: non-irradiated. Irradiation units: /cm2. *p < 0.05, **p < 0.01. Data are representative or mean ± s.e.m. of triplicate samples. See also Supplementary Figure 4
