Fig. 5: UV-induced squamous differentiation is independent of p53. | Cell Death & Disease

Fig. 5: UV-induced squamous differentiation is independent of p53.

From: Sublethal UV irradiation induces squamous differentiation via a p53-independent, DNA damage-mitosis checkpoint

Fig. 5

Primary human keratinocytes analysed 9 days after infections with the empty vector (CT) or with shRNA specific to p53 (shp53) 24 h (b, c) or 48 h (a) after a sbUV dose. a Representative flow cytometry light scatter analyses. Black boxes gate cells with high light scatter. Bar histogram displays the percent of cells with high scatter as indicated. b Expression of squamous differentiation markers keratin K1, keratin K10, filaggrin or involucrin (Invol) as measured by RT-qPCR 24 h after irradiation. c Western blotting of cells as in (b) on insoluble (Invol, K13 and K16; same number of cells per lane) or soluble (Filaggrin and GAPDH as its loading control) cellular protein fractions. NI: non-irradiated. *p < 0.05. Data are representative or mean ± s.e.m. of duplicate samples

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