Fig. 2: Compound KYZ3 inhibited STAT3 activity. | Cell Death & Disease

Fig. 2: Compound KYZ3 inhibited STAT3 activity.

From: RETRACTED ARTICLE: A new synthetic derivative of cryptotanshinone KYZ3 as STAT3 inhibitor for triple-negative breast cancer therapy

Fig. 2

a Western blot analysis of p-STAT3 (Tyr705) and STAT3 in whole-cell lysates of equal total protein prepared from MDA-MB-231 and MDA-MB-468 cells treated with compound KYZ3 for 24 h. b MDA-MB-231 and MDA-MB-468 cells were treated with KYZ3. For immunofluorescent assay, cells were fixed and stained with anti-phospho-STAT3 (p-STAT3) and DAPI before subjected to ImageXpress Micro Confocal analysis. Red or orange: p-STAT3; blue: nuclei; scale bar 20 μm. c Western blotting assay and d qPCR analysis of the levels of Bcl-2 and Bax in MDA-MB-231 and MDA-MB-468 cells treated with KYZ3 for 24 h ***p < 0.001

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