Fig. 4: Compound KYZ3 induced ROS generation without affecting the upstream kinases of STAT3.

a Western blot analysis of p-Src and p-Erk levels in whole-cell lysates of equal total protein prepared from MDA-MB-231 and MDA-MB-468 cells treated with compound KYZ3 for 24 h. MDA-MB-231 (b) and MDA-MB-468 (c) cells were incubated with KYZ3 at different concentrations for 24 h, and then cells were collected and stained by DCFH-DA and subjected to flow cytometry. ***p < 0.001, **p < 0.01