Fig. 6: ELK1 is essential for miR-139-3p to inhibit osteoblast differentiation and promote osteoblast apoptosis. | Cell Death & Disease

Fig. 6: ELK1 is essential for miR-139-3p to inhibit osteoblast differentiation and promote osteoblast apoptosis.

From: MicroRNA-139-3p regulates osteoblast differentiation and apoptosis by targeting ELK1 and interacting with long noncoding RNA ODSM

Fig. 6

Mimic-139 and pEX-ELK1 were co-transfected into MC3T3-E1 cells. a qRT-PCR analysis of osteoblast marker genes (Runx2, Bglap, Col1a1, and ALP normalized to GAPDH) mRNA levels in osteoblasts (N = 3). b Western blotting analyses of Runx2, Bglap, and Col1a1 protein expression in osteoblasts (N = 3). c ALP activity analysis in osteoblasts at 48 h (N = 3). d Representative images of ALP staining in osteoblasts (N = 3). e Flow cytometry analysis of apoptosis in osteoblasts stained with Annexin V-FITC/PI (N = 3). f, h Protein levels of Bax, Bcl-2, and cleaved caspase-3 in osteoblasts (N = 3). g Representative images of Hoechst 33258 staining in osteoblasts (N = 3). Scale bar, 50 µm. *P < 0.05, **P < 0.01 vs. control

Back to article page