Fig. 5: miR-27b regulated PAN-induced damage of cytoskeleton. | Cell Death & Disease

Fig. 5: miR-27b regulated PAN-induced damage of cytoskeleton.

From: MiR-27b regulates podocyte survival through targeting adenosine receptor 2B in podocytes from non-human primate

Fig. 5

a miR-27b overexpression enhanced PAN-induced cytoskeleton destruction. Cells were transfected with 50 nM miRNA mimic and treated with 10 μg/ml PAN for 3 days before fixation and staining. Scale: 50 µm. b Protein expression of nephrin was decreased by miR-27b. Cells were transfected with 50 nM miRNA mimic and treated with 10 µg/ml PAN for 3–5 days before collected and protein expression of nephrin was analyzed by western blotting. GAPDH was used as the loading control. c Inhibit the function of miR-27b by sponge ameliorated cytoskeleton damage induced by PAN. Scale bar: 50 µm. d Protein expression of nephrin was reversed by miR-27b sponge. Error bars represent data from three independent experiments. *p < 0.05 by Student's t-test

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