Fig. 1: miR-21 is induced by EGF and is required for EGF-induced pancreatic cancer cell proliferation.
From: miR-21 promotes EGF-induced pancreatic cancer cell proliferation by targeting Spry2
a The concentration-dependent effect of EGF on miR-21 level in PANC-1 cells. b The time-dependent effect of EGF on miR-21 level in PANC-1 cells. c miR-21 expression in other cell lines after 24 h of EGF treatment. d Relative expression of miR-21 in pancreatic cancer cell lines compared with normal pancreatic ductal epithelial cells. e After being serum-starved for 24 h, PANC-1 and MIA PaCa-2 cells were transfected with miR-21 mimics and inhibitor or negative control and simultaneously treated with or without EGF (50 ng/ml). CCK8 proliferation was performed to examine the growth of PANC-1 cells and MIA PaCa-2 cells. f EdU assay was used to further analyze the proliferative activity of PANC-1 cells and MIA PaCa-2 cells. Three independent experiments were performed for each group. All data are shown as the mean ± SD. *P <0.05; **P < 0.01; ***P < 0.001
