Fig. 2: H19 promotes 5-Fu resistance in CRC cells. | Cell Death & Disease

Fig. 2: H19 promotes 5-Fu resistance in CRC cells.

From: Long non-coding RNA H19 confers 5-Fu resistance in colorectal cancer by promoting SIRT1-mediated autophagy

Fig. 2

a The cytotoxic effect of 5-Fu in a panel of colorectal cell lines was measured by MTT assay after 72 h treatment with 5-Fu and IC50 value was calculated. b Relative expression of H19 in CRC cell lines and the normal colon epithelium cell line CCD-18Co by qRT-PCR. Data are presented as mean±SD from three independent experiments. c Dynamic changes of H19 expression level in response to 5-Fu treatment (0.1 μg/ml). The 1–8 time courses successively present 6, 12, 18, 24h after 5-Fu treatment and 30, 36, 42, 48h after medium replacement. d, h Validation of knockdown and overexpression efficacy of H19 in CRC cell line by qRT-PCR. ***P<0.001. e, f The cell sensitivity of HCT8Fu and SW1116 cells transfected with si-NC or si-H19, i, j HCT8 and HCT116 transfected with pcDNA3.1 or pcDNA-H19 to 5-Fu was evaluated using the MTT assay upon exposure to the step-up concentration of 5-Fu for 72h. **P<0.01, ***P<0.001. g, k Effects of H19 knockdown and overexpression on the IC50 value of cells was calculated. ***P<0.001

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