Fig. 3: LncATB functions by binding to miR-200 family members. | Cell Death & Disease

Fig. 3: LncATB functions by binding to miR-200 family members.

From: Long noncoding RNA ATB promotes the epithelial−mesenchymal transition by upregulating the miR-200c/Twist1 axe and predicts poor prognosis in breast cancer

Fig. 3

a RNA pull-down followed by microRNA qRT-PCR assays to detect miR-200 family members endogenously associated with lncATB. b MCF-7 cell lysates were incubated with biotin-labelled lncATB; after the pull-down, miR-200 miRNAs were extracted and assessed by qRT-PCR assays. c−g Relative luciferase activity in MCF-7 cells transfected with specific miR-200 luciferase reporters in the presence of pcDNA3.1-ATB alone or with the cotransfection of c miR-200a mimics, d miR-200b mimics, e miR-200c mimics, f miR-141 mimics, or g miR-429 mimics. h−l Relative luciferase activity in BT-549 cells cotransfected with specific miR-200 luciferase reporters and shATB-#1/2. For each specific reporter, h miR-200a inhibitor, i miR-200b inhibitor, j miR-200c inhibitor, k miR-141 inhibitor, or l miR-429 inhibitor was also added. *P < 0.05 and **P < 0.01

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