Fig. 4: Regulation of Twist1 mediated by lncATB via binding to miR-200c. | Cell Death & Disease

Fig. 4: Regulation of Twist1 mediated by lncATB via binding to miR-200c.

From: Long noncoding RNA ATB promotes the epithelial−mesenchymal transition by upregulating the miR-200c/Twist1 axe and predicts poor prognosis in breast cancer

Fig. 4

a Luciferase activity in MCF-7 cells cotransfected with luciferase reporter (pMIR-Vector, pMIR-Twist1-WT, or pMIR-Twist1-mut) and miR-200c mimics with a dose dependent. b Luciferase activity in BT-549 cells cotransfected with luciferase reporter (pMIR-Vector, pMIR-Twist1-WT, or pMIR-Twist1-mut) and miR-200c inhibitor with a dose dependent. c The mRNA levels of Twist1 in MCF-7 cells overexpressing lncATB or ectopically expressing both lncATB and miR-200c. d The mRNA levels of Twist1 in BT-549 cells with downregulated lncATB or with both shATB and miR-200c inhibitor. e The protein levels of Twist1 and E-Cad in MCF-7 cells overexpressing lncATB or ectopically expressing both lncATB and miR-200c. f The protein levels of Twist1 and Vimentin in BT-549 cells with downregulated lncATB or with both shATB and miR-200c inhibitor. *P < 0.05 and **P < 0.01

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