Fig. 6: LncATB deletion reduced breast cancer progression in vitro and in vivo. | Cell Death & Disease

Fig. 6: LncATB deletion reduced breast cancer progression in vitro and in vivo.

From: Long noncoding RNA ATB promotes the epithelial−mesenchymal transition by upregulating the miR-200c/Twist1 axe and predicts poor prognosis in breast cancer

Fig. 6

a Cell viability assay in BT-549 cells with knocked down lncATB. b Colony numbers were counted in the BT-549-shATB and control BT-549-shNC cell groups. c Wound-healing assay to evaluate the effect of lncATB on cell migration in the BT-549-shATB and control BT-549-shNC cell groups. d The migrated (left) and invaded (right) cell numbers in the groups of control BT-549-shNC cells, BT-549-shATB cells, and cells with both lncATB and miR-200c inhibition were counted 24 h after seeding. e For each mouse, shNC cells and lncATB-depleted cells were subcutaneously implanted into the left and right fourth mammary pad, respectively. There were two groups: shNC/shATB-#1 and shNC/shATB-#2, each group has three mice. f Tumours were measured every 3 days with external callipers, and the tumour volume was calculated according to the following formula: Volume = 0.5 × a2 × b, where ‘a’ is the smallest superficial diameter and ‘b’ is the largest superficial diameter. g Twenty-seven days after the tumour was implanted, the tumours were excised and weighed. Scale bar = 50 μm. *P < 0.05 and **P < 0.01

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