Fig. 4
From: Loss of the candidate tumor suppressor ZEB1 (TCF8, ZFHX1A) in Sézary syndrome
A Flow cytometry analysis showing the levels of ROS produced in ZEB1wt control cells and ZEB1 KO clones following exposure to 3.5 mU/mL and 4.5 mU/mL of GOX concentration; ROS are detected as H2DCFDA fluorogenic dye intensity. B The amount of ROS produced in ZEB1 KO clones, relative to the WT control, following 3.5 and 4.5 mU/mL of GOX treatment for 4 h. Data are expressed as means ± SEM (n = 3), *p < 0.05 **p < 0.01 vs. control. C MTT assay showing the differences in cell viability between the ZEB1wt control cells and ZEB1 KO clones treated with increasing concentration of GOX; *p < 0.05, **p < 0.001 vs. control. D Bar graph quantifying the percentage of dead and living cell sub-populations in ZEB1wt control and KO clones according to the indicated treatments. Data are expressed as percentage of total cell numbers. Light-gray bars indicate Annexin V-positive apoptotic cells
