Fig. 2: UBIAD1 regulates H-Ras trafficking from the Golgi apparatus to the plasma membrane. | Cell Death & Disease

Fig. 2: UBIAD1 regulates H-Ras trafficking from the Golgi apparatus to the plasma membrane.

From: UBIAD1 suppresses the proliferation of bladder carcinoma cells by regulating H-Ras intracellular trafficking via interaction with the C-terminal domain of H-Ras

Fig. 2

a UBIAD1 increased H-Ras retention in the Golgi apparatus in HEK293T cells. HEK293T cells were transfected with RFP-H-Ras with or without UBIAD1-GFP, followed by 24 h of culturing with or without cycloheximide for 5 h and confocal analysis. Histograms show the percentage of H-Ras localized in the Golgi. **p < 0.01 compared to control, (n = 3 experiments, each with 100 cells). b Ectopic expression of UBIAD1 increased the localization of Ras in the Golgi apparatus. T24 cells were transfected with or without UBIAD1-GFP, followed by 24 h of culture, stained with pan-Ras antibody and analyzed by confocal microscopy. Histograms show the percentage of Ras localized in the Golgi. **p < 0.01 compared to control, (n = 3 experiments, each with 100 cells). c Ras localized in the plasma membrane after knockdown of UBIAD1. HEK293T cells were transfected with sh-UBIAD1, followed by 72 h of culturing, staining with DiI (a maker of membrane), pan-Ras antibody and DAPI and confocal analysis. Histograms show the percentage of Ras localized in the plasma membrane. **p < 0.01 compared to control, (n = 3 experiments, each with 100 cells)

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