Fig. 8: IL-33 signal promotes MCs survival and function in vitro. | Cell Death & Disease

Fig. 8: IL-33 signal promotes MCs survival and function in vitro.

From: Mast cells are essential intermediaries in regulating IL-33/ST2 signaling for an immune network favorable to mucosal healing in experimentally inflamed colons

Fig. 8

a Representative plots of apoptosis assays of MCs treated with or without IL33 in the absence of SCF and IL-3. b Mean apoptosis percentage. Data were pooled from three repeated experiments. c Proliferation rate of MCs treated with or without IL-33 in the absence of SCF and IL-3, divided by results from regulate culture conditions (medium with IL-3 and SCF). d MC degranulation assessed by β-hexosaminidase release. Concentrations of e IL-13 and f IL-22 in supernatants of MCs, T cells or MCs co-cultures with T cells, by ELISA. g Percentage of IL-22/IL-13-coproducing T cells, IL-13-producing T cells, and IL-22-producing T cells in proliferating T cells by cell trace CFSE or nonproliferating cells assessed by flow cytometry. Plots used data from three repeated experiments. Data are mean ± SD. Student t-test, *P < 0.05; **P < 0.01

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