Fig. 2: Immunofluorescence microscopy exploring the association of mTOR to lysosomes, following drug exposure. | Cell Death & Disease

Fig. 2: Immunofluorescence microscopy exploring the association of mTOR to lysosomes, following drug exposure.

From: Lysosomotropic drugs activate TFEB via lysosomal membrane fluidization and consequent inhibition of mTORC1 activity

Fig. 2

U2OS cells were incubated with the indicated drugs for 3 hr and then subjected to immunofluorescence microscopy as detailed under Materials and methods. The localization of mTOR (green fluorescence) was followed together with LAMP1 (red fluorescence) which was used as a lysosomal marker. The right row depicts blow-ups of areas from the merged photos showing the (dis)association of mTOR and LAMP1. Nuclei were stained with the blue DNA dye Hoechst 33342. Cells were visualized by scanning confocal microscopy at a ×63 magnification. All fields are representative of at least three independent experiments

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