Fig. 4: Lysosomotropic drugs activate calcineurin, resulting in dephosphorylation of its substrate Elk-1. | Cell Death & Disease

Fig. 4: Lysosomotropic drugs activate calcineurin, resulting in dephosphorylation of its substrate Elk-1.

From: Lysosomotropic drugs activate TFEB via lysosomal membrane fluidization and consequent inhibition of mTORC1 activity

Fig. 4

a U2OS cells were treated with cyclosporin A (10 µM), siramesine (10 µM), or sunitinib (10 µM) for 4 hr. Nuclear proteins were extracted from the cells, and WB analysis was performed using Elk-1 and phospho-Elk-1 (Ser383)-specific antibodies. A β-actin-specific antibody was used to verify equal loading. b Band intensity was quantified using ImageJ software. Phosphorylation levels were determined by dividing the level of phospho-Elk-1 by the levels of total Elk-1 for each treatment. *p value < 0.05, **p value < 0.01—two-tailed Student's t test

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