Fig. 5: Inhibition of calcineurin does not prevent drug-induced nuclear translocation of TFEB. | Cell Death & Disease

Fig. 5: Inhibition of calcineurin does not prevent drug-induced nuclear translocation of TFEB.

From: Lysosomotropic drugs activate TFEB via lysosomal membrane fluidization and consequent inhibition of mTORC1 activity

Fig. 5

a U2OS-TFEB-eGFP cells were preloaded with BAPTA-AM (10 µM, 30 min), washed and incubated with siramesine (10 µM), sunitinib (10 µM), or chloroquine (100 µM) for an additional 4 hr. b U2OS-TFEB-eGFP cells were co-treated with or without the calcineurin inhibitors CsA (10 µM), FK-506 (5 µM), or both, as well as with siramesine (10 µM), sunitinib (10 µM), or chloroquine (100 µM). TFEB-eGFP was visualized using an InCell Analyzer fluorescence microscope

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