Fig. 7: Isobologram analysis of the combined impact of Torin-1 and siramesine on nuclear translocation of TFEB. | Cell Death & Disease

Fig. 7: Isobologram analysis of the combined impact of Torin-1 and siramesine on nuclear translocation of TFEB.

From: Lysosomotropic drugs activate TFEB via lysosomal membrane fluidization and consequent inhibition of mTORC1 activity

Fig. 7

U2OS-TFEB-eGFP cells were treated for 3 hr with increasing concentrations of Torin-1 (1.2–8.4 nM) or siramesine (2.4–16.8 µM) and the combinations of the two. TFEB-eGFP was visualized using an InCell Analyzer fluorescence microscope. The ratio between nuclear and cytoplasmic TFEB-eGFP levels was determined by staining nuclei with Hoechst 33342 (2 µg/ml) prior to drug exposure, and image analysis by InCell investigator software. Dashed lines indicate the line of additivity for each effect (relative TFEB nuclear levels of 0.5, 0.6, 0.7, and 0.8). Full lines indicate experimental results of the drug combinations for the same effect level. Error bars indicate standard deviation

Back to article page