Fig. 3: Effects of DR-Ab on glutamate-induced intrinsic membrane excitability and synaptic transmission in the primary cultured cortical neurons.

a Pulse protocol showing that 35-pA current step from −25 to 70 pA were used to evoke action potential. b Representative APs in neurons before and after a brief application of glutamate in DR or NS pretreated neurons. c Group data showing that DR-Ab abolished the glutamate-induced increase in spike numbers. n = 9/group for left, n = 10/group for right. Two-way repeated ANOVA followed by Bonferroni’s test, *p < 0.05. DR: DR-Ab; Glu: glutamate (100 µM) + glycine (10 μΜ) for 30 s; NS: IgG purified from normal rat serum. d–h Representative tracings (d) and group data (e-h) showing a glutamate-induced an increase in mEPSC frequency (f), and left shift of the cumulative probability for mEPSC inter-event interval (e), but no effects were observed on the cumulative probability for mEPSC amplitude (g, h). DR-Ab abolished the effect of glutamate on mEPSC frequency (e, f). n = 8/group. e and g: Kolmogorov–Smirnov Test, f and h: Unpaired t test, two-tailed, *p < 0.05. DR: DR-Ab; Glu: glutamate (100 µM) + glycine (10 μΜ) for 30 s; NS: IgG purified from normal rat serum